RNA
Lock 1

Part:BBa_J01010:Design

Designed by: Golden Bear   Group: iGEM2005   (2005-11-05)

Riboregulator Lock 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

m-fold file for the cr12 riboregulator

The part sequence contains a ribosome binding site with a short complementary sequence upstream. When this piece is transcribed into mRNA it forms a stem-loop structure which "locks" the ribosome binding site. This folded mRNA structure is known as the 'cis-repressed' part. Part is also available with a constituitively on promoter as BBa_J01060


Source

References

  • Isaacs, Dwyer DJ, Ding C, Pervouchine DD, Cantor CR, Collins JJ. 2004. "Engineered riboregulators enable post-transcriptional control of gene expression". Nature Biotechnology 2004. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15208640&query_hl=7&itool=pubmed_docsum Pubmed] [http://www.nature.com/nbt/journal/v22/n7/abs/nbt986.html;jsessionid=ABB92F041E994E3B8E4A5213699C3CB0 Journal]